Why should I use 0.1% formic acid to elute samples from Protein A ready for GlyX with InstantQ, rather than a low pH glycine solution?-ProZyme公司询价-蚂蚁淘生物试剂代购

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Why should I use 0.1% formic acid to elute samples from Protein A ready for GlyX with InstantQ, rather than a low pH glycine solution?

Glycine has a primary amine which will react with the InstantQ label, so we suggest that 0.1% formic acid is used as a protein eluent. Alternatively, glycine may be used but samples must be desalted before deglycosylation.

2018-01-12

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